Hyperactivation and Acrosome Reaction in Vitro in Spermatozoa Ejaculated by Cryptorchid Dogs after Orchiopexy.
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چکیده
منابع مشابه
Pentoxifylline induces hyperactivation and acrosome reaction in spermatozoa of golden hamsters: changes in motility kinematics.
Pentoxifylline (PF) is used to enhance motility of spermatozoa from infertile human subjects. We have previously shown that 0.45 mM PF improved capacitation of spermatozoa and fertilization of oocytes in vitro in hamsters. The present study was carried out to assess PF-induced changes in motility kinematics of hamster spermatozoa by a computer-aided sperm analyser (CASA) and determine the timin...
متن کاملDevelopmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملStimulation of in vitro activation and the acrosome reaction of hamster spermatozoa by catecholamines.
Capacitation and the acrosome reaction of mammalian spermatozoa are essential for fertilization. In vitro results are presented that demonstrate that catecholamines stimulate activation (a whiplash flagellar movement characteristic of capacitated hamster spermatozoa) and the acrosome reaction. Protein-free ultrafiltrates of bovine adrenal cortex and medulla preparations stimulated motility, act...
متن کاملEffect of in vitro incubation on spontaneous acrosome reaction in fresh and cryopreserved human spermatozoa.
OBJECTIVES Capacitation and acrosome reaction are prerequisites for fertilization. However, in vitro capacitation is not necessary for an agonist-induced acrosome reaction. We studied whether in vitro capacitation is important in spontaneous acrosome reaction and analyzed how capacitation before cryopreservation influences the acrosomal status of thawed spermatozoa. METHODS Semen specimens fr...
متن کاملEvaluation of lipid changes in buffalo (Bubalus bubalis) spermatozoa during in-vitro capacitation and acrosome reaction
In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1991
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.53.447